What Is Flow Cytometry?
lFlow ~ cells in motionlCyto ~ celllMetry ~ measurelMeasuring properties of cells while in a fluid stream lMany wavelengths of light will be scattered from a cell, we need a way to split the light into its specific wavelengths in order to detect them independently. This is done with filterslOptical filters are designed such that they absorb or reflect some wavelengths of light, while transmitting other.l3 types of filtersØLong Pass filterØShort Pass filterØBand Pass filter lCan be a long pass or short pass filterlFilter is placed at a 45º angle to the incident lightlPart of the light is reflected at 90º to the incident light, and part of the light is transmitted and continues on.